Nonviral Mammalian Expression Systems

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miRNA Expresion Vectors

Cellutron is providing you with most powerful miRNA tools to examine the effects of miRNAs on cellular function and phenotype. There are hundreds of inventoried vectors for overexpression of native miRNAs and antagomiR of your choice. Also, Cellutron has constructed expression vectors designed to express artificial miRNAs that are engineered to have 100% homology to your target gene and thus block gene expression.

Cellutron miRNA expression vectos allow allows you to:

1. Express artificial miRNA to knockdown the expression of target genes.

2. Transfect with high efficiency in variety of cell types including: dividing, non-diving cells, adult and embryonic stem cells and iPS cells.

3. Conditionally express miRNAs of your choice in vivo and in vitro.

4. Generate stable cell lines for long-term miRNA expression.

General miRNA pathway

MicroRNAs (miRNAs) are small, 21- to 25-nucleotide-long, noncoding RNAs that regulate gene expression by base pairing with the 3′ untranslated regions and probably also with the coding sequences of target mRNAs, leading to translational repression or degradation.

miRNAs are transcribed by RNA polymerase II and can be derived from individual miRNA genes, from introns of protein-coding genes, or from polycistronic transcripts that often encode multiple, closely related miRNAs. Pri-miRNAs, generally several thousand bases long, are processed in the nucleus by the RNase Drosha into 70–100 nucleotide long hairpin-shaped precursors, called pre-miRNAs. Following transport to the cytoplasm, pre-miRNA is further processed by the RNA endonuclease Dicer to produce a double-stranded miRNA.

This miRNA duplex is then incorporated into a multicomponent protein complex known as RNA-induced silencing complex (RISC). During this process, one strand of the miRNA duplex is selected as the mature miRNA, while the other strand is in general rapidlyremoved and degraded. Nucleotides 2–8 of the 5′ end of the miRNA were named “seed sequence” because this region seems to be specifically important for mRNA target identification.

MiRNAs are well conserved in eukaryotic organisms and play a vital role in gene expression and regulation.