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Adult Mouse Cardiomyocyte Perfusion

 

 

   

    

         Perfusion Flow Rate:

  • Adult mouse heart: 3 - 4 ml / min

 

 
   

The optimal tubing length should hold 12 ~ 13ml solution. Please adjust it before using our cell isolation system.

 
     

1.

 

2.

 
3. 4.

 

After perfusion, cut and digest the tissue as follows:

   

 
   

1. Perfusion

2. After perfusion, cut the tissue off

          from perfusion apparatus

 
   

 

 

 
   

3. Cut the tissue

4. Cut the tissue into small pieces

 
   

 

 

 
   

5. Put the tissue pieces into 50 ml tube

6. Add 5 - 6 ml enzyme solution in the tube and shaking horizontally on a shaker .

 
   

 

 

 

For technical questions, please contact:

techsupport@cellutron.com

 

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7. Use 5 ml pipette with cut tip

and pipet the tissue up and down

 

   
         
 

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Cellutron Life Technology, 2007